Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoids.

ABSTRACT: Human neural organoid models have become an important tool for studying neurobiology. However, improving the representativeness of neural cell populations in such organoids remains a major effort. In this work, we compared Matrigel, a commercially available matrix, to a neural cadherin (N-cadherin) peptide-functionalized gelatin methacryloyl hydrogel (termed GelMA-Cad) for culturing cortical neural organoids. We determined that peptide presentation can tune cell fate and diversity in gelatin-based matrices during differentiation. Of particular note, cortical organoids cultured in GelMA-Cad hydrogels mapped more closely to human fetal populations and produced neurons with more spontaneous excitatory postsynaptic currents relative to Matrigel. These results provide compelling evidence that m

SUBMITTER: Kjar A 

PROVIDER: S-EPMC11682736 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature