Prolonged storage reduces viability of <i>Peptacetobacter (Clostridium) hiranonis</i> and core intestinal bacteria in fecal microbiota transplantation preparations for dogs.

ABSTRACT:

Introduction

Fecal microbiota transplantation (FMT) has been described useful as an adjunct treatment for chronic enteropathy in dogs. Different protocols can be used to prepare and store FMT preparations, however, the effect of these methods on microbial viability is unknown. We aimed (1) to assess the viability of several core intestinal bacterial species by qPCR and (2) to assess Peptacetobacter (Clostridium) hiranonis viability through culture to further characterize bacterial viability in different protocols for FMT preparations.

Methods

Bacterial abundances were assessed in feces from six healthy dogs by qPCR after propidium monoazide (PMA-qPCR) treatment for selective quantitation of viable bacteria. Conservation methods tested included lyophiliza

SUBMITTER: Correa Lopes B 

PROVIDER: S-EPMC11747423 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature