M1A-regulated DIAPH3 promotes the invasiveness of colorectal cancer via stabilization of KRT19.

ABSTRACT:

Background

In recent years, the emphasis has shifted to understanding the role of N1-methyladenosine (m1A) in tumor progression as little is known about its regulatory effect on mRNA and its role in the metastasis of colorectal cancer (CRC).

Methods

We performed methylated RNA immunoprecipitation sequencing of tumor tissues and tumor-adjacent normal tissues from three patients with CRC to determine the m1A profile of mRNA in CRC. The expression of diaphanous-related formin 3 (DIAPH3) and its correlation with clinicopathological characteristics of CRC were evaluated using immunohistochemistry and online datasets. The role of DIAPH3 in the migration and invasion of CRC cells was evaluated using wound healing assay, Transwell assay and xenograft metastatic model. The downstream

SUBMITTER: Mi S 

PROVIDER: S-EPMC11754336 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature