A tunable and reversible thermo-inducible bio-switch for streptomycetes.

ABSTRACT: Programmable control of bacterial gene expression holds great significance for both applied and academic research. This is particularly true for Streptomyces, a genus of Gram-positive bacteria and major producers of prodigious natural products. Despite that a few inducible regulatory systems have been developed for use in Streptomyces, there is an increasing pursuit to augment the toolkit of high-performance induction systems. We herein report a robust and reversible thermo-inducible bio-switch, designated as StrepT-switch. This bio-switch enables tunable and reversible control of gene expression using physiological temperatures as stimulation inputs. It has been proven successful in highly efficient CRISPR/Cas9-mediated genome engineering, as well as programmable control of antibiotic pro

SUBMITTER: Lv L 

PROVIDER: S-EPMC11754652 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature