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New insights into metabolic properties of marine bacteria encoding proteorhodopsins.


ABSTRACT: Proteorhodopsin phototrophy was recently discovered in oceanic surface waters. In an effort to characterize uncultured proteorhodopsin-exploiting bacteria, large-insert bacterial artificial chromosome (BAC) libraries from the Mediterranean Sea and Red Sea were analyzed. Fifty-five BACs carried diverse proteorhodopsin genes, and we confirmed the function of five. We calculate that proteorhodopsin-exploiting bacteria account for 13% of microorganisms in the photic zone. We further show that some proteorhodopsin-containing bacteria possess a retinal biosynthetic pathway and a reverse sulfite reductase operon, employed by prokaryotes oxidizing sulfur compounds. Thus, these novel phototrophs are an unexpectedly large and metabolically diverse component of the marine microbial surface water.

SUBMITTER: Sabehi G 

PROVIDER: S-EPMC1175822 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature

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New insights into metabolic properties of marine bacteria encoding proteorhodopsins.

Sabehi Gazalah G   Loy Alexander A   Jung Kwang-Hwan KH   Partha Ranga R   Spudich John L JL   Isaacson Tal T   Hirschberg Joseph J   Wagner Michael M   Béjà Oded O  

PLoS biology 20050719 8


Proteorhodopsin phototrophy was recently discovered in oceanic surface waters. In an effort to characterize uncultured proteorhodopsin-exploiting bacteria, large-insert bacterial artificial chromosome (BAC) libraries from the Mediterranean Sea and Red Sea were analyzed. Fifty-five BACs carried diverse proteorhodopsin genes, and we confirmed the function of five. We calculate that proteorhodopsin-exploiting bacteria account for 13% of microorganisms in the photic zone. We further show that some p  ...[more]

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