Detection of Iron Protein Supercomplexes in <i>Pseudomonas aeruginosa</i> by Native Metalloproteomics.

ABSTRACT: Pseudomonas aeruginosa is a major contributor to human infections and is widely distributed in the environment. Its ability for growth under aerobic and anaerobic conditions provides adaptability to environmental changes and to confront immune responses. We applied high-throughput native 2-dimensional metalloproteomics to P. aeruginosa to examine how use of iron within the metallome responds to oxic and anoxic conditions. Metalloproteomic analyses revealed four major iron peaks, each comprised of metalloproteins with synergistic functions, including: 1) respiratory and metabolic enzymes, 2) oxidative stress response enzymes, 3) DNA synthesis and nitrogen assimilation enzymes, and 4) denitrification enzymes and related copper enzymes. Three ferritins co-eluted with the first a

SUBMITTER: Saito MA 

PROVIDER: S-EPMC11760780 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature