Structural Characterization of Foxtail Millet (<i>Setaria italica</i>) Polysaccharides and Evaluation of Its Antioxidant and Immunostimulatory Activities.

ABSTRACT: A water-soluble polysaccharide from foxtail millet (FM-D1) was isolated and purified through gradient ethanol precipitation and column chromatography. Size-Exclusion Chromatography-Multi-Angle Light Scattering-Refractive Index (SEC-MALLS-RI) and high-performance anion-exchange chromatography (HPAEC) analyses revealed that FM-D1 constitutes a highly purified neutral polysaccharide exclusively composed of glucose as the sugar unit, with a molecular weight of 14.823 kDa. The structural characterization results obtained from gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance spectroscopy (NMR) spectra suggest that FM-D1 primarily consists of a main chain linked by →4)-α-D-Glcp-(1→ and minor quantities of →4,6)-α-D-Glcp-(1→ to form the main chain, with bra

SUBMITTER: Zhang H 

PROVIDER: S-EPMC11761630 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature