Identification and Candidate Gene Analysis of <i>Brcl1</i>, a Novel Gene Confers a Leaf Curled Phenotype in <i>Brassica rapa</i> L.

ABSTRACT: Leaf shape is an important determinant of photosynthesis, yield and quality in plants. In this study, we obtained a curled leaf mutant, cl1, from an ethyl methanesulfonate (EMS)-induced mutagenesis population. It was designated the Brcl1^YS locus. Bulk segregant RNA sequencing combined with recombinant screening identified the candidate interval responsible for Brcl1^YS in a 97.5 kb region on chromosome A02. Twelve genes were identified within the candidate region. Sequence differences and co-separation verification confirmed that BraA02g017030.3C was the most promising candidate gene underlying the Brcl1^YS locus. It is homologous to Arabidopsis AT1G66350 (RGL1), which has been shown to act as a negative regula

SUBMITTER: Wang L 

PROVIDER: S-EPMC11765633 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature