A <i>Clostridioides difficile</i> cell-free gene expression system for prototyping and gene expression analysis.

ABSTRACT: Clostridioides difficile is an obligate anaerobic, Gram-positive bacterium that produces toxins. Despite technological progress, conducting gene expression analysis of C. difficile under different conditions continues to be labor-intensive. Therefore, there is a demand for simplified tools to investigate the transcriptional and translational regulation of C. difficile. The cell-free gene expression (CFE) system has demonstrated utility in various applications, including prototyping, protein production, and in vitro screening. In this study, we developed a C. difficile CFE system capable of in vitro transcription and translation (TX-TL) in the presence of oxygen. Through optimization of cell extract preparation and reaction systems, we increased the

SUBMITTER: Zeng J 

PROVIDER: S-EPMC11784378 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature