A DNA Methylation Signature From Buccal Swabs to Identify Tuberculosis Infection.

ABSTRACT:

Background

Tuberculosis (TB) is among the largest infectious causes of death worldwide, and there is a need for a time- and resource-effective diagnostic methods. In this novel and exploratory study, we show the potential of using buccal swabs to collect human DNA and investigate the DNA methylation (DNAm) signatures as a diagnostic tool for TB.

Methods

Buccal swabs were collected from patients with pulmonary TB (n = 7), TB-exposed persons (n = 7), and controls (n = 9) in Sweden. Using Illumina MethylationEPIC array, the DNAm status was determined.

Results

We identified 5644 significant differentially methylated CpG sites between the patients and controls. Performing the analysis on a validation cohort of samples collected in Kenya and Peru (patients, n = 26; exposed,

SUBMITTER: Karlsson L 

PROVIDER: S-EPMC11793033 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature