METTL3 modulates chromatin and transcription dynamics during cell fate transition.

ABSTRACT: Transcriptional programming plays a key role in determining the cell state. Timely reconfiguration of chromatin structure and attenuation of pluripotent genes are required for efficient embryonic stem cell (ESC) differentiation. Here, we identify METTL3, a core N⁶-methyladenosine (m⁶A) catalyzing enzyme, as a crucial modulator of dynamic transcription and chromatin accessibility upon ESC-derived cardiac differentiation. Genome-wide analysis of chromatin-associated RNAs revealed that depletion of METTL3 failed to dramatically attenuate the transcription of pluripotent genes, as well as activate nascent cardiomyocyte-specific transcripts upon differentiation. Consistently, ATAC-seq analysis showed that loss of METTL3 markedly attenuated the dynamic alteration of chromat

SUBMITTER: Liu XM 

PROVIDER: S-EPMC11803071 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature