Metabolic Engineering of Filamentous Fungus <i>Trichoderma reesei</i> for Itaconic Acid Production.

ABSTRACT: Itaconic acid (IA) is a multifunctional platform chemical with numerous biological functions. Here, Trichoderma reesei was engineered as a remarkable cell factory to produce IA. Heterologous overexpression of the mitochondrial tricarboxylate transport protein and cis-aconitate decarboxylase from Aspergillus terreus in T. reesei initiated IA production with a titer of 20 g/L. By increasing the copy number of mttA and cadA and the overexpression of the plasma membrane transporter proteins (MFSA), the titer of IA reached 56.7 g/L. The precursor synthesis pathway of IA was overexpressed by the overexpression of aconitase and citrate synthase, and the IA competition pathway was blocked by the deletion of the P450 monooxygenase gene cyp3 to furthe

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11869998 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature