Wastewater surveillance for Salmonella Typhi and its association with seroincidence of enteric fever in Vellore, India.

ABSTRACT:

Background

Blood culture-based surveillance for typhoid fever has limited sensitivity, and operational challenges are encountered in resource-limited settings. Environmental surveillance targeting Salmonella Typhi (S. Typhi) shed in wastewater (WW), coupled with cross-sectional serosurveys of S. Typhi-specific antibodies estimating exposure to infection, emerges as a promising alternative.

Methods

We assessed the feasibility and effectiveness of wastewater (WW) and sero-surveillance for S. Typhi in Vellore, India, from May 2022 to April 2023. Monthly samples were collected from 40 sites in open drainage channels and processed using standardized protocols. DNA was extracted and analyzed via quantitative PCR for S. Typhi genes (ttr, tviB, staG) and the fecal biomarker HF183. C

SUBMITTER: Abraham D 

PROVIDER: S-EPMC11896026 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature