TGF-β1 Mediates Novel-m0297-5p Targeting <i>WNT5A</i> to Participate in the Proliferation of Ovarian Granulosa Cells in Small-Tailed Han Sheep.

ABSTRACT: MiRNAs regulate follicle development and atresia, steroid production, granulosa cell (GC) proliferation, and apoptosis. However, the target genes and the functioning of novel miRNAs remain unexplored. We reveal the targeting relationship between novel-m0297-5p and WNT5A and the specific regulatory mechanism of GC proliferation in small-tailed Han sheep using whole transcriptomic sequencing. We performed whole transcriptomic sequencing on small-tailed Han sheep ovarian GCs supplemented with 10 ng/mL of transforming growth factor-β1 (TGF-β1) during the early stages. This led to identifying the differential expression of novel-m0297-5p and Wnt family member 5A (WNT5A) and predicting their targeting relationship. Based on this, we hypothesized that TGF-β1 could mediate novel-m029

SUBMITTER: Ren S 

PROVIDER: S-EPMC11901034 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature