Cloning and Functional Analysis of Glyoxalase I Gene <i>BrGLYI 13</i> in <i>Brassica rapa</i> L.

ABSTRACT: Glyoxalase I (GLYI) is a key enzyme that detoxifies methylglyoxal, a toxic byproduct of glycolysis, and is essential for plant pollination. However, the genome-wide identification and functional analysis of GLYI in Brassica rapa L. (B. rapa) remain limited. This study identified 17 BrGLYI genes (BrGLYI1-BrGLYI17) from the B. rapa genome. The self-compatible line 039-1 and the self-incompatible line GAU-28-5 were used as experimental materials, and Real-Time Quantitative Reverse Transcription PCR (RT-qPCR) was performed to examine the effect of BrGLYI genes on self-compatibility in winter B. rapa. Preliminary results showed that BrGLYI13 exhibited significant tissue specificity, with higher expression in the flowers of 03

SUBMITTER: Song X 

PROVIDER: S-EPMC11942965 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature