Genomic Functional Analysis and Cellulase Characterization for the Enzyme-Producing Strain <i>Bacillus subtilis</i> Y4X3 Isolated from Saline-Alkaline Soil in Xinjiang, China.

ABSTRACT: Biotechnological research and application of microbial enzyme production have consistently been focal points for scientific inquiry and industrial advancement. In this study, Bacillus subtilis Y4X3 was isolated from saline-alkaline soil in Xinjiang, China. Extracellular enzyme production analysis revealed that B. subtilis Y4X3 can secrete various enzymes, including cellulase, xylanase, protease, and amylase. Sequencing and assembly of the complete genome of this strain revealed a genome size of 4,215,636 bp with 43.51% C + G content, including 4438 coding genes. Genome annotation was performed with databases to predict gene functions in B. subtilis Y4X3, and a variety of genes related to carbohydrate metabolism were identified. A cellulase-encoding gene was subsequentl

SUBMITTER: Yao X 

PROVIDER: S-EPMC11944486 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature