Excited-State-Altering Ratiometric Fluorescent Probes for the Response of <i>β</i>-Galactosidase in Senescent Cells.

ABSTRACT: β-galactosidase (β-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development of a fluorescent sensor is of considerable importance for precisely detecting the activity and spatial distribution of β-Gal. In this study, we developed two excited-state-altering responsive fluorescent sensors (TF1 and TF2) for ratiometric detection of β-Gal. Two TCF dyes, composed of tricyanofuran (TCF) and naphthol units, feature electron "pull-push" systems and are quenched fluorescence by β-Gal. Upon β-Gal hydrolysis, a significant ratiometric shift in absorption from ca. 475 nm to 630 nm is observed, accompanied by the emergence of a fluorescence signal at ca. 660 nm. Th

SUBMITTER: Han YN 

PROVIDER: S-EPMC11945828 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature