Size-exclusion chromatography-small-angle neutron scattering system optimized for an instrument with medium neutron flux.

ABSTRACT: Biomacromolecular solutions inevitably contain impurities in addition to the target biomacromolecules. This has been a major obstacle to achieving high-precision solution scattering measurements. To overcome this problem, small-angle X-ray scattering (SAXS) coupled with size-exclusion chromatography (SEC-SAXS) has been developed. This method involves injecting the solution eluted by SEC directly into a measurement cell and conducting SAXS measurements during the elution of the target biomacromolecule. This technique has resulted in a paradigm shift in biomacromolecule solution scattering. Currently, the application of the SEC-SAXS system to small-angle neutron scattering (SANS) is being advanced. However, since the target biomacro-mol-ecules in the sample solution are not only purified but

SUBMITTER: Morishima K 

PROVIDER: S-EPMC11957415 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature