The first complete mitochondrial genome assembly and comparative analysis of the fern Blechnaceae family: <i>Blechnopsis orientalis</i>.

ABSTRACT:

Introduction

Blechnopsis orientalis (L.) C. Presl is a medicinal and edible fern species belonging to the Blechnaceae family. Currently, the complete mitochondrial genome of B. orientalis, as well as those of other Blechnaceae species, remains unreported, and studies on fern mitochondrial genome are limited.

Methods

In this study, the B. orientalis mitochondrial genome was sequenced using both Nanopore PromethION and Illumina NovaSeq 6000 platforms. Genome annotation was performed using MITOFY and MFANNOT, with structural visualization via OGDRAW. In-depth analyses were conducted, including assessments of non-synonymous/synonymous mutation ratios (Ka/Ks), codon usage bias, repeat sequence identification, RNA editing site prediction, collinearity, and the

SUBMITTER: Huang Y 

PROVIDER: S-EPMC11965612 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature