Immunostimulatory effects mechanism of polysaccharide extracted from Acanthopanax senticosus on RAW 264.7 cells through activating the TLR/MAPK/NF-κB signaling pathway.

ABSTRACT: Acanthopanax senticosus is a unique wild resource in Northeast China. Its main active ingredient is a polysaccharide, which has a prominent immunomodulatory effect. In this study, the purified polysaccharide component of Acanthopanax senticosus leaves(ASPS-A1) with strong immunomodulatory activity was isolated by column chromatography, and its structure and properties were characterized by HPGPC, and FT-IR. Results showed that ASPS-A1 is mainly composed of α- 1,4-_D-GalA, α- 1,5-_L-Ara, and β- 1,4-_D-Gal. RT-qPCR experiments and RNA-seq analysis were used to study the immunoregulatory mechanism of ASPS-A1. The results showed that ASPS-A1 could significantly up-regulate the levels of cytokines iNOS, IL- 1β, IL- 6, and TNF-α activated macrophages through MAPK, N

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12008293 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature