Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model.

ABSTRACT:

Background

Induced pluripotent stem cells (iPSCs) can propagate indefinitely and give rise to every other cell type, rendering them invaluable for disease modelling, drug development research, and usage in regenerative medicine. While feline iPSCs have been described, there are currently no reports on generating genome integration (footprint)-free iPSCs from domestic cats. Therefore, the objective of this study was to generate feline iPSCs from fetal fibroblasts using non-integrative Sendai virus (SeV) vectors carrying human transcription factors. Moreover, these iPSCs were differentiated into mesenchymal stromal cells (MSCs), which can be used as an alternative to tissue-derived MSCs.

Methods

Feline fetal fibroblasts were transduced with CytoTune-iPS 2.0 Sendai Reprogrammin

SUBMITTER: Weeratunga P 

PROVIDER: S-EPMC12010622 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature