Cloning, bioinformatics analysis, and expression of the ubiquitin 2 (ubq-2) gene from the dog roundworm <i>Toxocara canis</i>.

ABSTRACT: Toxocariasis, caused by the dog roundworm Toxocara canis, is a globally distributed zoonotic parasitic disease that poses a significant threat to veterinary and public health. The ubiquitin plus an L40 ribosomal protein (UBQ-2) in parasites plays a crucial role in protein degradation and meiotic divisions, thereby affecting parasite development, survival, and parasite-host interactions. In this study, we identified for the first time a full-length cDNA encoding the UBQ-2 protein from the T. canis-omic dataset, called Tcubq-2. After cloning and sequencing, we conducted sequence analysis and structural modeling of TcUBQ-2 using online bioinformatics tools. The recombinant TcUBQ-2 protein (rTcUBQ-2) was prokaryotically expressed and subjected to Western blot analysis to assess i

SUBMITTER: Zhu P 

PROVIDER: S-EPMC12010969 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature