UV-C and hydration state drive pulsed light-induced proteome damage in <i>Bacillus pumilus</i> spores.

ABSTRACT:

Introduction

Pulsed light (PL) is a non-thermal processing technology that inactivates microorganisms through high-intensity pulses of polychromatic light, including ultraviolet-C (UV-C). While the germicidal effect of PL has been widely studied, its impact on microbial proteomes remains poorly understood. Here, we investigate the proteomic response of Bacillus pumilus DSM492 (ATCC 27142) spores to PL treatment, comparing it to conventional UV-C 254 nm exposure.

Methods

B. pumilus spores were either suspended in water or sprayed onto a polystyrene surface and exposed to PL or UV-C at fluences achieving a 5-log and a > 7-log reduction in viability. Proteomic changes were analyzed using mass spectrometry to identify proteins with decreased abundance after treatme

SUBMITTER: Dorbani I 

PROVIDER: S-EPMC12017682 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature