Single cell RNA sequencing of haematopoietic cells in fresh and frozen human atheroma tissue.

ABSTRACT:

Aims

Single-cell RNA sequencing (scRNA-seq) is a powerful method for exploring the cellular heterogeneity within human atheroma but typically requires fresh tissue to preserve cell membrane integrity, limiting the feasibility of large-scale biobanking for later analysis. The aim of this study was to determine whether cryopreservation of fragile and necrotic atheroma tissue affects the viability and transcriptomic profiles of haematopoietic cells in subsequent scRNA-seq analysis, enabling the use of cryopreserved atheroma samples for future research.

Methods and results

We performed scRNA-seq on five paired fresh and cryopreserved atheroma samples-three from coronary arteries and two from carotid arteries. Each sample was enzymatically digested, sorted for CD45+ haematopoieti

SUBMITTER: Ahmad H 

PROVIDER: S-EPMC12038232 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature