Novel Porcine Model Reveals Two Distinct LGR5 Cell Types during Lung Development and Homeostasis.

ABSTRACT: Cells expressing leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) play a pivotal role in homeostasis, repair, and regeneration in multiple organs, including skin and gastrointestinal tract, but little is known about their role in the lung. Findings from mice, a widely used animal model, suggest that lung LGR5 expression differs from that of humans. In this work, using a new transgenic pig model, we identify two main populations of LGR5⁺ cells in the lung that are conserved in human but not mouse lungs. Using RNA sequencing, three-dimensional imaging, and organoid models, we determine that in the fetal lung, epithelial LGR5 expression is transient in a subpopulation of SOX9⁺/ETV5⁺/SFTPC⁺ progenitor lung tip cells. In contrast, ep

SUBMITTER: Polkoff KM 

PROVIDER: S-EPMC12051919 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature