In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors.

ABSTRACT: MicroRNAs are essential regulators of gene expression. Their function is particularly important during neurogenesis, when the production of large numbers of neurons from a limited number of neural stem cells depends on the precise control of determination, proliferation and differentiation. However, microRNAs can target many mRNAs and vice-versa, raising the question of how specificity is achieved to elicit a precise regulatory response. Here we introduce in vivo AGO-APP, a novel approach to purify Argonaute-bound, and therefore active microRNAs from specific cell types. Using AGO-APP in the larval Drosophila central nervous system, we identify a module of microRNAs predicted to redundantly target all iconic genes known to control the transition from neuroblasts to neurons. While microRNA

SUBMITTER: Narbonne-Reveau K 

PROVIDER: S-EPMC12064045 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature