Distinct strategies of diguanylate cyclase domain proteins on inhibition of virulence and interbacterial competition by agrobacteria.

ABSTRACT: Diguanylate cyclases (DGCs) synthesize bis-(3',5')-cyclic diguanylic acid (c-di-GMP), a critical bacterial second messenger that coordinates diverse biological processes. Agrobacterium tumefaciens, a plant pathogen causing crown gall disease, relies on type IV secretion system for pathogenesis and type VI secretion system (T6SS) for interbacterial competition. Our study identified two putative DGCs, named diguanylate cyclase domain proteins regulating virulences A and B (DcvA and DcvB), that negatively regulate virulence through distinct mechanisms. DcvA suppresses virulence by targeting the VirA/VirG two-component system downstream of VirA. This inhibition is independent of c-di-GMP levels. DcvB positively regulates biofilm formation, inhibits T6SS-mediated int

SUBMITTER: Lai X 

PROVIDER: S-EPMC12077199 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature