<i>Drosophila</i> peptidyl-prolyl <i>cis/trans</i> isomerase-like 4 regulates circadian rhythm by supporting high-amplitude oscillations of PERIOD.

ABSTRACT: Peptidyl-prolyl cis/trans isomerases (PPIases) accelerate proline peptide bond isomerization, affecting substrate protein function. In this study, through RNAi-based behavioral screening of PPIases in Drosophila melanogaster, we identified CG5808, termed Drosophila peptidyl-prolyl cis/trans isomerase-like 4 (dPPIL4), as crucial for circadian rhythm regulation. Knockdown of dppil4 in clock cells lengthened the circadian rhythm period and decreased rhythmicity, accompanied by a significant reduction of core clock protein PERIOD (PER). d ppil4 knockdown downregulated per transcription and reduced phosphorylation at Ser5 in the RNA polymerase II C-terminal domain, critical for transcription elongation. In addition, dPPIL4 stabilize

SUBMITTER: Kang SW 

PROVIDER: S-EPMC12084006 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature