Toxoplasma calcium-dependent protein kinases 3 mediates M1 macrophage polarization by targeting host Arginase-1.

ABSTRACT:

Background

Toxoplasma gondii, an obligate intracellular parasite, has developed sophisticated ways to manipulate host immunity, resulting in long-lasting infection and causing serious public health problems in humans and animals. T. gondii type II is the type most frequently associated with human diseases, but the mechanism remains unclear. Toxoplasma calcium-dependent protein kinase 3(CDPK3), a protein located on the T. gondii parasite periphery, is highly expressed in type II strains. Although TgCDPK3 regulates parasite egress from host cells, calcium-based infiltration, and development of tissue cysts, the host target proteins that it modulates are still unclear.

Methods

Firstly, mass spectrometry was used to analyze proteins that selectively bind to TgCDPK3. Subsequently

SUBMITTER: An R 

PROVIDER: S-EPMC12090600 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature