Biochemical Characterization and Polyester-Binding/Degrading Capability of Two Cutinases from <i>Aspergillus fumigatus</i>.

ABSTRACT: Two recombinant cutinases, AfCutA and AfCutB, derived from Aspergillus fumigatus, were heterologously expressed in Pichia pastoris and systematically characterized for their biochemical properties and polyester-degrading capabilities. AfCutA demonstrated superior catalytic performance compared with AfCutB, displaying higher optimal pH (8.0-9.0 vs. 7.0-8.0), higher optimal temperature (60 °C vs. 50 °C), and greater thermostability. AfCutA exhibited increased hydrolytic activity toward p-nitrophenyl esters (C4-C16) and synthetic polyesters. Additionally, AfCutA released approximately 3.2-fold more acetic acid from polyvinyl acetate (PVAc) hydrolysis than AfCutB. Quartz crystal microbalance with dissipation monitoring (QCM-D) reve

SUBMITTER: Wang H 

PROVIDER: S-EPMC12114444 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature