Drosophila Clu ribonucleoprotein particle dynamics rely on the availability of functional Clu and translating ribosomes.

ABSTRACT: Drosophila Clu is a conserved multi-domain ribonucleoprotein essential for mitochondrial function that forms dynamic particles within the cytoplasm. Unlike stress granules and processing bodies (P-bodies), Clu particles disassemble under nutritional or oxidative stress. However, it is unclear how disrupting protein synthesis affects Clu particle dynamics, especially given that Clu binds mRNA and ribosomes. Here, we capitalize on ex vivo and in vivo imaging of Drosophila female germ cells to determine what domains of Clu are necessary for Clu particle assembly and how manipulating translation affects particle dynamics. Using domain deletion analysis, we identified three domains of Clu essential for particle assembly. We also demonstrated that overexpressing functional Clu led to disassembly

SUBMITTER: Hwang HJ 

PROVIDER: S-EPMC12136168 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature