Establishment and characterization of an inflammatory cartilaginous organoids model for organoid transplantation study.

ABSTRACT:

Background

Transplantation of cartilaginous organoids for repairing cartilage defects in osteoarthritis represents a novel treatment approach. However, A controversial argument remains about whether cartilaginous organoids derived from the differentiation of bone marrow mesenchymal stem cells (BMSCs) in the three-dimensional (3D) environment are strictly organoids and whether the inflammatory microenvironment would affect the success rate of organoid transplantation. This study characterized 3D BMSC-derived cartilaginous organoids and developed an inflammatory organoid model to better understand the transcriptomic changes in the organoids induced by the microenvironment when transplanted into the knee with osteoarthritis.

Methods

Spatial growth BMSCs were generated and cultu

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12138948 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature