Regulating Biocondensates within Synthetic Cells via Segregative Phase Separation.

ABSTRACT: Living cells orchestrate a myriad of biological reactions within a highly complex and crowded environment. A major factor responsible for such seamless assembly is the preferential interactions between the constituent macromolecules, that can drive demixing to produce coexisting phases and thus provide dynamic intracellular compartmentalization. However, the way multiple-phase separation phenomena, occurring simultaneously within the cytoplasmic space, influence each other is still largely unknown. Here, we show that the interplay between segregative and associative phase separation within cell-mimicking confinements can lead to rich dynamics between multiple phases and the lipid boundary. Using on-chip microfluidic systems, we encapsulate the associative and segregative components and ext

SUBMITTER: Chen C 

PROVIDER: S-EPMC12164530 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature