Hypotaurine Reduces Glucose-Mediated Vascular Calcification.

ABSTRACT:

Aim

Vascular calcification (VC), a characteristic feature of peripheral artery disease in patients with diabetes and chronic kidney disease, has been associated with poor prognosis. We hypothesize that hyperglycemia drives VC through alterations in metabolomic and transcriptomic profiles.

Methods

Human coronary artery smooth muscle cells (SMCs) were cultured with 0, 5.5, and 25 mM glucose under calcifying conditions. Untargeted metabolomic and transcriptomic analyses were performed at different time points. Mitochondrial respiration was examined using Seahorse analysis.

Results

Glucose-treated SMCs promoted extracellular matrix (ECM) calcification in a concentration- and time-dependent manner. The absence of glucose entirely abolished SMC calcification but reduced SMC

SUBMITTER: Heuschkel MA 

PROVIDER: S-EPMC12230643 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature