Swarm-seq Reveals Functional Divergence in CsrA Homologs Across Bacteria.

ABSTRACT: Bacteria employ sophisticated post-transcriptional regulatory mechanisms to adapt to environmental changes. Carbon storage regulator A (CsrA), a highly conserved small RNA-binding protein, serves as a critical post-transcriptional regulator by recognizing GGA motifs in target transcripts and typically repressing translation. Despite extensive research, how this conserved regulator has evolved diverse species-specific regulatory networks remains unclear. Here, using Bacillus subtilis as an experimental chassis, we have developed Swarm-seq - a high-throughput functional genomics platform capable of assessing CsrA homologs across the Bacterial domain for their ability to regulate flagella-dependent swarming motility. Systematically testing over five-hundred codon-optimized csrA

SUBMITTER: Winkelman JT 

PROVIDER: S-EPMC12247646 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature