Impaired envelope integrity in the absence of SanA is linked to increased Lipid II availability and an imbalance of FtsI and FtsW activities.

ABSTRACT: In gram-negative bacteria, the outer membrane (OM) acts in conjunction with the peptidoglycan (PG) cell wall as a barrier against physical, osmotic, and toxic environmental stresses including antibiotics. SanA, an inner membrane protein in Escherichia coli K-12, is required for vancomycin resistance at high temperatures (>42 °C) and we have previously demonstrated that SanA impacts sodium dodecyl sulfate (SDS) resistance during stationary phase reached from carbon limitation. However, its function remains unknown. Here, we show that ΔsanA has a synthetic genetic interaction with ΔwecA, a mutation increasing the availability of the isoprenoid carrier for PG biosynthesis. Specifically, the ΔsanA ΔwecA strain demonstrated heightened SDS EDTA sensitivity, Rcs

SUBMITTER: Carr JF 

PROVIDER: S-EPMC12259093 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature