Retrospective and multifactorial single-cell profiling reveals sequential chromatin reorganization during X inactivation.

ABSTRACT: The regulation of gene expression is governed at multiple levels of chromatin organization. However, how gene regulation is co-ordinated remains relatively unexplored. Here we develop Dam&ChIC, a method that enables retrospective and multifactorial chromatin profiling in single cells. Dam&ChIC employs chromatin labelling in living cells with ^m6A to acquire a past chromatin state, coupled with an antibody-mediated readout to capture the present chromatin state. Analyses of diverse factor combinations highlight its versatility and superior resolution. By tracking lamina-associated domain inheritance over the cell cycle, we showcase that Dam&ChIC provides retrospective single-cell chromatin data. When applied in random X chromosome inactivation, Dam&ChIC disentangles the temporal o

SUBMITTER: Kefalopoulou S 

PROVIDER: S-EPMC12270907 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature