A pilot study of healing critical-sized calvarial defects by LL-37-generated monoosteophils.

ABSTRACT:

Introduction

Monoosteophils, derived from LL-37-treated monocytes, are a novel type of calcifying/bone forming cells. We have shown that monoosteophils can form bone-like nodules in vitro and accelerate bone repair in a drilled femur defect model. Here, we explored the bone repair function of monoosteophils in a mouse model of critical-sized calvarial defect and the mechanism of bone nodule formation of monoosteophils in vitro.

Methods

Human monocytes were isolated from peripheral blood and differentiated into monoosteophils. Critical-sized (5 mm-diameter) calvarial defects in the parietal bone of adult male NOD/SCID mice were implanted with either 1-day untreated human monocytes, 1-day LL-37 treated human monocytes (monoosteophils), 1-day human monocytes plus

SUBMITTER: Le K 

PROVIDER: S-EPMC12301765 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature