Lactate inhibits mouse embryonic palatal mesenchyme cells chondrogenesis through AKT-PKM2 axis.

ABSTRACT:

Background

The development of the palate is a complex and continuous process, occurring in a series of stages with cartilage of being particular significance. Chondrogenesis is influenced by many factors such as metabolism, and hypoxia which are also risk factors for cleft palate. Hypoxia can increase the intracellular lactate levels due to increased glycolysis. However, the precise role of lactate in palatal cartilage development remains unclear.

Methods

Mouse embryonic palatal mesenchyme (MEPM) cells isolated from embryonic day 13.5 (E13.5) and E12.5 were obtained and characterized using immunofluorescence. Subsequently, following a 21-day induction culture in a chondrogenic medium, the MEPM cells were subjected to alcian blue staining and to quantitative real-time PCR to

SUBMITTER: Meng M 

PROVIDER: S-EPMC12315313 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature