From glomalin to glomalose: unraveling the molecular identity of the MAb32B11 antigen.

ABSTRACT: Glomalin, a substance produced by arbuscular mycorrhizal (AM) fungi, has well-documented benefits for plant and soil health, including water retention and soil aggregation. Glomalin quantification has been performed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody, MAb32B11, that has been described as targeting a heat shock protein 60 (RiHSP60). In this study, we re-examined the molecular nature of the antigen recognized by MAb32B11. MAb32B11 did not cross-react with the RiHSP60 polypeptide. Glomalin extracts of Rhizophagus irregularis showed strong and dose-dependent cross-reactivity with MAb32B11 even when protein levels were undetectable, raising doubts about the proteinaceous nature of the antigen. Protease treatments of glomalin extracts did not affect the ELIS

SUBMITTER: Alptekin B 

PROVIDER: S-EPMC12329162 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature