PLCγ2 controls neutrophil-like cell sensitivity through calcium oscillation and gates chemoattractant concentration range for chemotaxis.

ABSTRACT: The relationship between calcium oscillation and cell sensitivity is poorly understood. Calcium oscillation can occur spontaneously or be triggered upon receptor-ligand binding. The cytosolic [Ca²⁺] increase during calcium oscillation is initiated from Ca²⁺ release from the intracellular stores through the phospholipase C (PLC)-derived inositol 1,4,5-trisphosphate (IP₃). Here, we show that neutrophil-like HL60 cells lacking PLCγ2 (plcg2^kd ) exhibit impaired spontaneous calcium oscillation and a diminished calcium response to chemoattractant stimulation. These defects result in reduced membrane targeting of RasGAP CAPRI (calcium-promoted Ras inactivator), and subsequent elevated Ras activations and enhanced downstream signaling, including P

SUBMITTER: Xu X 

PROVIDER: S-EPMC12354650 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature