Low-Background His-Tag-Targeting Probes for Turn-On Fluorescence Detection of Cell Surface Proteins and Their Binding Interactions.

ABSTRACT: Turn-on fluorescent probes consisting of dye-ligand conjugates serve as a powerful tool for detecting cell surface proteins (CSPs) and their interactions with binding partners. However, generating such probes from protein-based ligands remains challenging. This challenge became particularly evident during the COVID-19 pandemic, which highlighted the need for assays to evaluate inhibitors of the interaction between the SARS-CoV-2 virus receptor-binding domain (RBD) and the angiotensin-converting enzyme 2 (ACE2) receptor. To sense this interaction in a cellular environment using turn-on probes, a tri-nitrilotriacetic acid (tri-NTA) unit was conjugated to quinoline-based cyanine (QBC) dyes. This design leverages the high affinity of tri-NTA for His-tag, along with the low-background and confi

SUBMITTER: Prasad PK 

PROVIDER: S-EPMC12372432 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature