Highly sensitive and label-free detection of SARS-CoV-2 proteins via surface plasmon resonance using biofunctionalization with 1 nm thick carbon nanomembranes.

ABSTRACT: Here we report a novel platform for the detection of nucleocapsid (N) and receptor-binding domain (RBD) of spike (S) proteins of SARS-CoV-2 viruses using the surface plasmon resonance (SPR) technique. We demonstrate that the functionalization of SPR sensors with molecular 2D materials - 1 nm thick carbon nanomembranes (CNMs) significantly enhances sensitivity. CNMs terminated with azide linker (N3-CNM) enable covalent bonding of SARS-CoV-2 antibodies for specific immobilization of the N- and S-proteins to the sensor surface. The successful and stable hierarchical functionalization is confirmed by multiparametric SPR measurements complemented with X-ray photoelectron spectroscopy and polarization modulation infrared reflection absorption spectroscopy. The obtained equilibrium dissociation c

SUBMITTER: Eshaghi G 

PROVIDER: S-EPMC12378216 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature