Insights from metagenomics on microbial biosynthesis of vitamins B and K₂ in chicken gut microbiota.

ABSTRACT:

Introduction

The chicken gut microbiome plays a pivotal role in nutrient absorption and overall health, contributing to the biosynthesis of essential vitamins. However, the biosynthesis of vitamins B and K₂ by the whole gut microbiome, as well as their abundances across different gut regions, remains largely unknown.

Methods

We employed both metagenomic sequencing and culture-based techniques, collecting a total of 25,825 genomes (25,764 metagenome-assembled genomes and 61 isolated genomes). After quality assessment and average nucleotide identity (ANI), 13,734 genomes were retained for downstream analysis.

Results

Whole-genome clustering analysis identified 2,675 species-level genome bins (SGBs), predominantly from the phyla Bacillota, Bacteroidota, Pse

SUBMITTER: Yu HL 

PROVIDER: S-EPMC12393000 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature