MALDI-TIMS-MS² Imaging and Annotation of Natural Products in Fungal-Bacterial Coculture.

ABSTRACT: Mass spectrometry imaging (MSI) is a powerful tool for monitoring the spatial distributions of microbial metabolites directly from culture. MSI can identify secretion and retention patterns for microbial metabolites, allowing for the assessment of chemical communication within complex microbial communities. Microbial imaging via matrix-assisted laser desorption/ionization (MALDI) MSI remains challenging due to high sample complexity and heterogeneity associated with the required sample preparation, making annotation of molecules by MS¹ alone challenging. The implementation of trapped ion mobility spectrometry (TIMS) has increased the dimensionality of MALDI-MSI experiments, allowing for the resolution of isomers and isobars, and can increase sensitivity of metabolite detection w

SUBMITTER: Shepherd RA 

PROVIDER: S-EPMC12413305 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature