MicroRNA-372-3p impairs fatty acid metabolism in hepatocellular carcinoma cells by targeting <i>CPT1A</i> and <i>ACSL4</i>.

ABSTRACT:

Introduction

Hepatocellular carcinoma (HCC) remains a major cause of cancer mortality, and effective therapeutic options are limited. MicroRNA‑372‑3p (miR‑372‑3p) has been implicated in HCC, yet its exact role is unclear.

Methods

We established miR‑372‑3p‑overexpressing HCC cell lines (HepG2, SNU‑449, JHH‑4) via lentiviral transduction. Malignant phenotypes were assessed with MTT, transwell migration/invasion, and colony‑formation assays. Transcriptomic changes were analyzed by RNA‑sequencing followed by Gene Set Enrichment Analysis. Lipid metabolism was examined using BODIPY/Oil Red O staining, triglyceride quantification, FAOBlue assays, and organelle colocalization imaging. Candidate targets of miR‑372‑3p were computationally predicted and validated by dual‑luciferase rep

SUBMITTER: Phetkong C 

PROVIDER: S-EPMC12413982 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature