GLP-1R signaling does not modify the severity of experimental graft versus host disease.

ABSTRACT:

Objective

Glucagon-like peptide-1 (GLP-1) reduces systemic and gut inflammation. Here we assessed whether gain or loss of GLP-1 receptor (GLP-1R) signaling modifies the extent of gut injury and inflammation in experimental murine acute graft vs. host disease (aGvHD).

Methods

Allogeneic hematopoietic cell transplantation (HCT) was performed using bone marrow and splenocytes from BALB/c donors to induce aGvHD in C57BL/6 recipients or vice versa. Chimerism was determined by flow cytometry analysis of immune cell compartments. Inflammation was assessed by histological scoring of gut mucosal damage and by measuring circulating cytokine levels. qPCR was used to quantify gene expression in small intestine immune cells and tissues. The gut microbiome was assessed by 16S rRNA sequenc

SUBMITTER: Yusta B 

PROVIDER: S-EPMC12414834 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature