Engrafted NSG-SGM3 humanized mice spontaneously produce human immunoglobulins including IgE.

ABSTRACT: NSG-SGM3 humanized mouse models are well-suited for studying human immune physiology but are technically challenging and expensive. We previously characterized a simplified NSG-SGM3 mouse, engrafted with human donor CD34⁺ hematopoietic stem cells without receiving prior bone marrow ablation or human secondary lymphoid tissue implantation, that still retains human mast cell- and basophil-dependent passive anaphylaxis responses. Its capacities for human antibody production and human B cell maturation, however, remain unknown. Here, we show that NSG-SGM3 mice engrafted without prior marrow ablation spontaneously produce all human antibodies, including IgE, without deliberate sensitization. These human IgE antibodies are polyclonal with unexpected specificities to diverse allergens,

SUBMITTER: Lin EV 

PROVIDER: S-EPMC12414937 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature