Validating a Target-Enrichment Design for Capturing Uniparental Haplotypes in Ancient Domesticated Animals.

ABSTRACT: In the last three decades, DNA sequencing of ancient animal osteological assemblages has become an important tool complementing standard archaeozoological approaches to reconstruct the history of animal domestication. However, osteological assemblages of key archaeological contexts are not always available or do not necessarily preserve enough ancient DNA for a cost-effective genetic analysis. Here, we develop an in-solution target-enrichment approach, based on 80-mer species-specific RNA probes (ranging from 306 to 1686 per species) to characterise (in single experiments) the mitochondrial genetic variation from eight domesticated animal species of major economic interest: cattle, chickens, dogs, donkeys, goats, horses, pigs and sheep. We also illustrate how our design can be adapted to e

SUBMITTER: More KD 

PROVIDER: S-EPMC12415940 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature