Magnetite Nanoparticles Enhancing H₂-Driven Biomethanation in a Mixed Microbial Community.

ABSTRACT: Biological methanation is increasingly considered for biogas upgrading. Here, the supplementation of conductive magnetite (Fe₃O₄) nanoparticles is investigated as a strategy to enhance H₂-driven biomethanation in a mixed hydrogenotrophic methanogenic community. An enrichment culture, maintained for over 180 days in a fill-and-draw anaerobic bioreactor under H₂/CO₂ feeding, is used to inoculate batch microcosms containing 0, 1.25, and 2.5 gFe L^-1 of magnetite. Magnetite addition resulted in a dose-dependent increase in maximum methane production rates-up to 13-fold compared to controls-and sustained high hydrogen-to-methane conversion yields (78-107%). 16S rRNA gene sequencing reveals that archaeal community composition remai

SUBMITTER: Tucci M 

PROVIDER: S-EPMC12418360 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature